human retina marathon ready cdna library  (TaKaRa)

Journal: Human molecular genetics

Article Title: A novel TEAD1 mutation is the causative allele in Sveinsson's chorioretinal atrophy (helicoid peripapillary chorioretinal degeneration).

doi: 10.1093/hmg/ddh106

Figure Lengend Snippet: Figure 7. RT–PCR detection of TEAD1 and YAP65 expression in human tissues by nested primers resulted in one to two clear bands on agarose gel. The templates used were four Marathon-Ready cDNA libraries from Clontech (He, heart; Pa, pancreas; Br, brain; and Re, retina), cDNA from two cell lines (Ly, EBV lymphocytes; and Bl, whole blood) and human genomic DNA (Neg) as negative control. Both genes are expressed in the six cDNA libraries and not in the negative control. TEAD1 expression analysis results in only one band of the expected size 490 bp. YAP65 expected RNA product was 717 bp, detected in all except in the RNA extracted from blood where we detected two splice variants, one smaller and the other larger than the expected product.

Article Snippet: The primary and nested primers were designed to confirm the transcripts for TEAD1 and YAP65 in the four Marathon-Ready cDNA libraries from Clontech [He: Human Heart MarathonReady cDNA (7404-1); Pa: Human Pancreas Marathon-Ready cDNA (7410-1); Br: Human Brain, whole Marathon-Ready cDNA (7400-1); and Re: Human Retina Marathon-Ready cDNA (7449-1)], the two deCODE cDNA libraries (EBV transformed lymphocytes and whole blood) in addition to the negative control (human whole blood DNA).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Agarose Gel Electrophoresis, Negative Control